Overview of automated immunohistochemistry.

@article{Prichard2014OverviewOA,
  title={Overview of automated immunohistochemistry.},
  author={Jeffrey W. Prichard},
  journal={Archives of pathology \& laboratory medicine},
  year={2014},
  volume={138 12},
  pages={
          1578-82
        }
}
  • J. Prichard
  • Published 26 November 2014
  • Biology
  • Archives of pathology & laboratory medicine
CONTEXT The increasing demand for immunohistochemistry for clinical diagnostics, in combination with an ongoing shortage of staff in the histology laboratory, has brought about a need for automation in immunohistochemistry. The current automated staining platforms vary significantly in their design and capabilities. OBJECTIVE To review how technology has been applied to automating the process of immunohistochemical staining. DATA SOURCES Literature review, vendor interviews, and personal… 
Overview of automated immunohistochemistry.
  • J. Prichard
  • Biology
    Archives of pathology & laboratory medicine
  • 2014
TLDR
Each of the commercially available, automated immunohistochemistry platforms has strategic design differences that produce advantages and disadvantages and understanding those differences can help match the demands of testing volumes, turnaround time, standardization, and labor savings to the appropriate automated instrumentation.
Effects of Automation on Sustainability of Immunohistochemistry Laboratory
TLDR
Automation of the IHC procedure reduces the time and costs of theIHC process, contributing significantly to the sustainability of the healthcare system during the COVID-19 pandemic, overcoming insufficient human resources.
Uneven Staining in Automated Immunohistochemistry: Cold and Hot Zones and Implications for Immunohistochemical Analysis of Biopsy Specimens
TLDR
The common occurrence of UES (particularly decreased staining) has important implications for the reliable read-out of IHC assays on biopsy samples and baseline and periodic quality assurance testing for UES is recommended for all automated IHC instruments.
An Update on Immunohistochemistry in Translational Cancer Research
TLDR
An overview on the current status of IHC as applied in translational cancer research is provided, commenting on the underlying principles in specimen preparation, reagent choice, staining procedure, and results evaluation so that both beginners and seasoned users could appreciate the key factors and benefit from this update.
Next-generation protein analysis in the pathology department
TLDR
The workflow, advantages and drawbacks of both MALDI IMS and IHC are discussed, as well as a few other protein imaging modalities and give examples of applications.
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TLDR
An attempt is made to evaluate the phytochemical properties of the H2O/O2 complex, which has the potential to provide clues to the origin of infectious disease.
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TLDR
The microfluidic matching of Ab staining intensity using three HER2 Abs produced by different manufacturers was characterized and the quality of HER2 Ab was validated using tissues of breast cancer patients, demonstrating that the system is an efficient and powerful tool for the standardization and quality assurance of IHC.
Tissue Multiplex Analyte Detection in Anatomic Pathology – Pathways to Clinical Implementation
TLDR
Until mIF reagents, digital pathology systems including fluorescence scanners, and data pipelines are standardized, it is proposed that diagnostic labs will play a crucial role in driving adoption of multiplex tissue diagnostics by using retrospective data from tissue collections as a foundation for laboratory-developed test (LDT) implementation and use in prospective trials as companion diagnostics (CDx).
An Audit of Failed Immunohistochemical Slides in a Clinical Laboratory: The Role of On-Slide Controls
TLDR
In the era of automated IHC staining platforms, on-slide controls allow for the proper identification of IHC slides that should be failed by the IHC laboratory and represent a powerful tool for preventing the reporting of false-negative/false-positive tests.
Fully automated 5-plex fluorescent immunohistochemistry with tyramide signal amplification and same species antibodies.
TLDR
This technology enables automated mIHC using unmodified 1°Abs from same species and the corresponding anti-species 2°Ab on a clinically established automated platform to ensure staining quality, reliability and reproducibility.
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Overview of automated immunohistochemistry.
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  • Biology
    Archives of pathology & laboratory medicine
  • 2014
TLDR
Each of the commercially available, automated immunohistochemistry platforms has strategic design differences that produce advantages and disadvantages and understanding those differences can help match the demands of testing volumes, turnaround time, standardization, and labor savings to the appropriate automated instrumentation.
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