Overview of CRISPR-Cas9 Biology.

  title={Overview of CRISPR-Cas9 Biology.},
  author={Hannah K. Ratner and Timothy R. Sampson and David S Weiss},
  journal={Cold Spring Harbor protocols},
  volume={2016 12}
Prokaryotes use diverse strategies to improve fitness in the face of different environmental threats and stresses, including those posed by mobile genetic elements (e.g., bacteriophages and plasmids). To defend against these elements, many bacteria and archaea use elegant, RNA-directed, nucleic acid-targeting adaptive restriction machineries called CRISPR… CONTINUE READING