Overproduction of glycolytic enzymes in yeast.

  title={Overproduction of glycolytic enzymes in yeast.},
  author={Ine Schaaff and J{\"u}rgen Heinisch and Friedrich K. Zimmermann},
  volume={5 4},
Eight different enzymes for glycolysis and alcoholic fermentation were overproduced in a common Saccharomyces cerevisiae strain by placing their genes on multicopy vectors. The specific enzyme activities were increased between 3.7- and 13.9-fold above the wild-type level. The overproduction of the different glycolytic enzymes had no effect on the rate of… CONTINUE READING