Overproduction of beta-glucosidase in active form by an Escherichia coli system coexpressing the chaperonin GroEL/ES.

@article{Machida1998OverproductionOB,
  title={Overproduction of beta-glucosidase in active form by an Escherichia coli system coexpressing the chaperonin GroEL/ES.},
  author={Sachiko Machida and Y Yu and Satya Praksh Singh and Jong Deog Kim and Kiyoshi Hayashi and Yasushi Kawata},
  journal={FEMS microbiology letters},
  year={1998},
  volume={159 1},
  pages={41-6}
}
beta-Glucosidase from Cellvibrio gilvus was successfully overproduced in soluble form in Escherichia coli, with the coexpression of GroEL/ES. Without the GroEL/ES protein, the beta-glucosidase overexpressed in E. coli constituted a huge amount (80%) of the total cellular protein, but was localized in the insoluble fraction, and little activity was detected in the soluble fraction. Coexpression of the E. coli GroEL/ES had a drastic impact on the proper folding of the beta-glucosidase; 20% of the… CONTINUE READING