Overproduction of Microbial Transglutaminase in Escherichia coli, In Vitro Refolding, and Characterization of the Refolded Form

@article{Yokoyama2000OverproductionOM,
  title={Overproduction of Microbial Transglutaminase in Escherichia coli, In Vitro Refolding, and Characterization of the Refolded Form},
  author={K. Yokoyama and N. Nakamura and K. Seguro and K. Kubota},
  journal={Bioscience, Biotechnology, and Biochemistry},
  year={2000},
  volume={64},
  pages={1263 - 1270}
}
The Streptoverticillium transglutaminase (MTG) gene, synthesized previously for yeast expression, was modified and resynthesized for overexpession in E. coli. A high-level expression plasmid, pUCTRPMTG-02(+), was constructed. Furthermore, to eliminate the N-terminal methionine, pUCTRPMTGD2 was constructed. Cultivation of E. coli transformed with pUCTRPMTG-02(+) or pUCTRPMTGD2 yielded a large amount of MTG (200~300 mg/liter) as insoluble inclusion bodies. The N-terminal amino acid residue of the… Expand
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