Overproduction and purification of recombinant Bacillus subtilis RNA polymerase.

@article{Yang2008OverproductionAP,
  title={Overproduction and purification of recombinant Bacillus subtilis RNA polymerase.},
  author={Xiao Yang and Peter J Lewis},
  journal={Protein expression and purification},
  year={2008},
  volume={59 1},
  pages={86-93}
}
We describe a vector-based system for the production of recombinant Bacillus subtilis RNA polymerase. The recombinant enzyme is C-terminally tagged with nine consecutive histidine residues resulting in about 90% pure enzyme in a single nickel-affinity purification step. The vectors permitted production of recombinant enzyme lacking an omega subunit or containing either the omega(1) (YkzG) or omega(2) (YloH) subunits. In transcription time-course assays all of the recombinant enzymes exhibited… CONTINUE READING

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