Overproduction and characterization of the iclR gene product of Escherichia coli K-12 and comparison with that of Salmonella typhimurium LT2.

@article{Negre1991OverproductionAC,
  title={Overproduction and characterization of the iclR gene product of Escherichia coli K-12 and comparison with that of Salmonella typhimurium LT2.},
  author={Didier Neg̀re and J C Cortay and Iain G. Old and Anne Galinier and C Richaud and Isabelle Saint Girons and Alain J. Cozzone},
  journal={Gene},
  year={1991},
  volume={97 1},
  pages={29-37}
}
The iclR gene of Escherichia coli K-12, which encodes a regulatory protein (repressor) for the aceBAK operon, is located between that operon and metH in the 91-min region of the chromosome. The iclR gene was cloned and expressed in a coupled T7 RNA polymerase/promoter system and the gene product was identified by specific binding to a fragment containing the aceBAK operator region. The iclR gene product is a polypeptide of 274 amino acids (aa) with a calculated Mr of 29,741. Comparison of the… CONTINUE READING

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