Overproduction, crystallization and preliminary X-ray analysis of the putative L-ascorbate-6-phosphate lactonase UlaG from Escherichia coli.

@article{Garces2008OverproductionCA,
  title={Overproduction, crystallization and preliminary X-ray analysis of the putative L-ascorbate-6-phosphate lactonase UlaG from Escherichia coli.},
  author={Fernando Garces and F. J. Fern{\'a}ndez and Rosa P{\'e}rez-Luque and Juan Aguilar and Laura Baldom{\`a} and M. Dolores Coll and Josefa Bad{\'i}a and Maria Cristina Vega},
  journal={Acta crystallographica. Section F, Structural biology and crystallization communications},
  year={2008},
  volume={64 Pt 1},
  pages={36-8}
}
UlaG, the putative L-ascorbate-6-phosphate lactonase encoded by the ulaG gene from the utilization of L-ascorbate regulon in Escherichia coli, has been cloned, overexpressed, purified using standard chromatographic techniques and crystallized. Crystals were obtained by sitting-drop vapour diffusion at 293 K. Preliminary X-ray diffraction analysis revealed… CONTINUE READING