Overexpression ofRNaseH partially complements thegrowth defect ofan Escherichia coliAtopAmutant:R-loopformation isa majorproblemintheabsenceofDNA topoisomerase I

@inproceedings{Phoenix1995OverexpressionOP,
  title={Overexpression ofRNaseH partially complements thegrowth defect ofan Escherichia coliAtopAmutant:R-loopformation isa majorproblemintheabsenceofDNA topoisomerase I},
  author={Pauline Phoenix},
  year={1995}
}
Previous biochemical studies havesuggested a roleforbacterial DNA topoisomerase (TOPO)Iinthe suppression ofR-loop formation during transcription. Inthis report, wepresent several pieces ofgenetic evidence tosupport amodelinwhichR-loop formation isdynamically regulated during transcription byactivities ofmultiple DNATOPOsand RNaseH.Inaddition, ourresults suggest that events leading totheserious growth problems intheabsence ofDNATOPO Iarelinked toR-loop formation. We showthattheoverex- pression… CONTINUE READING

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