Overexpression of the acidic c-terminus region of hvraf from barley confers enhanced cadmium tolerance in yeast

Abstract

The barley ERF-type transcription factor, HvRAF, has an acidic C-terminus domain that contains a hemopexin domain signature-like sequence. As an initial study to investigate the functioning of this domain, we cloned the partialHvRAF gene(pHvRAF) corresponding to the domain into a yeast/E.coli shuttle vector, pYES2. We then introduced thepYES2::pHvRAF recombinant plasmid into yeastSaccharomyces cerevisiae strain INVSd ). Here, we report that yeast transformants harboring pYES2::pHvRAF show significant tolerance to cadmium stress.

DOI: 10.1007/BF03030672

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Cite this paper

@article{Jung2009OverexpressionOT, title={Overexpression of the acidic c-terminus region of hvraf from barley confers enhanced cadmium tolerance in yeast}, author={Jinwook Jung and Minkyun Kim}, journal={Journal of Plant Biology}, year={2009}, volume={50}, pages={383-385} }