Overexpression of recombinant human antithrombin III in Chinese hamster ovary cells results in malformation and decreased secretion of recombinant protein.

@article{Schroeder1997OverexpressionOR,
  title={Overexpression of recombinant human antithrombin III in Chinese hamster ovary cells results in malformation and decreased secretion of recombinant protein.},
  author={Markus Schroeder and Peter Friedl},
  journal={Biotechnology and bioengineering},
  year={1997},
  volume={53 6},
  pages={547-59}
}
Overexpression of recombinant proteins in animal cells is commonly achieved by using gene amplification techniques. Gene amplified cells possess up to several thousand genes coding for the target protein. Constitutive expression of these genes leads to high levels of the corresponding mRNA species and the immature protein in the cell. Inefficient processing of these precursors may result from their great abundance in the cell. To study the influence of elevated intracellular levels of a… CONTINUE READING

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