Overexpression of pucC improves the heterologous protein expression level in a Rhodobacter sphaeroides expression system.

@article{Cheng2015OverexpressionOP,
  title={Overexpression of pucC improves the heterologous protein expression level in a Rhodobacter sphaeroides expression system.},
  author={Longcong Cheng and Guobao Chen and Gengzhi Ding and Zhiping Zhao and T. T. Bich Dong and Zongli Hu},
  journal={Genetics and molecular research : GMR},
  year={2015},
  volume={14 2},
  pages={4058-67}
}
The Rhodobacter sphaeroides system has been used to express membrane proteins. However, its low yield has substantially limited its application. In order to promote the protein expression capability of this system, the pucC gene, which plays a crucial role in assembling the R. sphaeroides light-harvesting 2 complex (LH2), was overexpressed. To build a pucC overexpression strain, a pucC overexpression vector was constructed and transformed into R. sphaeroides CQU68. The overexpression efficiency… CONTINUE READING