Overexpression of nef as a marker for restricted HIV-1 infection of astrocytes in postmortem pediatric central nervous tissues.

@article{Saito1994OverexpressionON,
  title={Overexpression of nef as a marker for restricted HIV-1 infection of astrocytes in postmortem pediatric central nervous tissues.},
  author={Yuji Saito and Leroy R. Sharer and Leon G. Epstein and Jennifer R. Michaels and Mark Mintz and Mark Louder and K Golding and Therese A. Cvetkovich and Benjamin M Blumberg},
  journal={Neurology},
  year={1994},
  volume={44 3 Pt 1},
  pages={474-81}
}
In previous studies, using polymerase chain reaction amplification of HIV-1 genes directly from pathologic tissues of children who died with AIDS encephalopathy, we showed that the reading frame of the HIV-1 regulatory nef gene is open, suggesting that the nef protein was expressed. We now show, using immunocytochemistry and in situ hybridization with nef-specific probes in postmortem pediatric CNS tissues, that nef mRNA and protein are present in up to 20% of astrocytes in tissue sections… CONTINUE READING
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