Overexpression of mitogen-activated protein kinase kinase (MAPKK) and its mutants in NIH 3T3 cells. Evidence that MAPKK involvement in cellular proliferation is regulated by phosphorylation of serine residues in its kinase subdomains VII and VIII.

@article{Seger1994OverexpressionOM,
  title={Overexpression of mitogen-activated protein kinase kinase (MAPKK) and its mutants in NIH 3T3 cells. Evidence that MAPKK involvement in cellular proliferation is regulated by phosphorylation of serine residues in its kinase subdomains VII and VIII.},
  author={Rony Seger and Dalia Seger and Alfred A. Reszka and Enric Munar and Hagit Eldar-Finkelman and Grażyna Dobrowolska and Anne Marya Jensen and Jean S. Campbell and Edmond Henri Fischer and Edwin G. Krebs},
  journal={The Journal of biological chemistry},
  year={1994},
  volume={269 41},
  pages={25699-709}
}
Mitogen-activated protein kinase kinase (MAPKK) is a dual specificity protein kinase that exhibits a high degree of specificity toward its downstream target, mitogen-activated protein kinase (MAPK). In this study, we used stable overexpression of MAPKK and its mutants in NIH 3T3 cells to study effects on downstream components of the MAPK signaling cascade and to correlate them to physiological responses. We have mutated the potential regulatory serine residue 222 to alanine (S222A) or to… CONTINUE READING