Overexpression of human transforming growth factor-beta1 using a recombinant CHO cell expression system.

@article{Zou2004OverexpressionOH,
  title={Overexpression of human transforming growth factor-beta1 using a recombinant CHO cell expression system.},
  author={Zhongcheng Zou and Peter D. Sun},
  journal={Protein expression and purification},
  year={2004},
  volume={37 2},
  pages={265-72}
}
Transforming growth factor-beta1 (TGF-beta1) is secreted by most cells as a high molecular weight latent complex, which consists of latent TGF-beta1 disulfide bonded to latent TGF-beta1-binding protein (LTBP). Current recombinant expression systems yield less than 1-2 mg of the mature TGF-beta1 per liter of cell culture medium. In an effort to produce large quantities of the recombinant cytokine for structural studies, we have constructed a mammalian expression system based on a modified pcDNA3… CONTINUE READING

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