Overexpression of human prostaglandin G/H synthase-1 and -2 by recombinant vaccinia virus: inhibition by nonsteroidal anti-inflammatory drugs and biosynthesis of 15-hydroxyeicosatetraenoic acid.

@article{ONeill1994OverexpressionOH,
  title={Overexpression of human prostaglandin G/H synthase-1 and -2 by recombinant vaccinia virus: inhibition by nonsteroidal anti-inflammatory drugs and biosynthesis of 15-hydroxyeicosatetraenoic acid.},
  author={G. Patrick O'Neill and Joseph A. Mancini and Stacia Kargman and James L Yergey and Matthew F. Y. Kwan and J. P. Falgueyret and Mark Abramovitz and Brian P. Kennedy and Marc Ouellet and Wanda A Cromlish},
  journal={Molecular pharmacology},
  year={1994},
  volume={45 2},
  pages={245-54}
}
Human prostaglandin G/H synthase (hPGHS)-1 and hPGHS-2, key enzymes in the formation of prostanoids from arachidonic acid, were expressed at high levels in COS-7 cells using a T7 RNA polymerase/vaccinia virus expression system. The open reading frame of hPGHS-2 cloned into vaccinia virus without its natural 5' and 3' untranslated regions directed only low levels of hPGHS-2 enzyme activity in COS-7 cells. High-level hPGHS-2 expression was achieved by appending the 3' untranslated region of hPGHS… CONTINUE READING
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