Overexpression of groESL in Clostridium acetobutylicum results in increased solvent production and tolerance, prolonged metabolism, and changes in the cell's transcriptional program.

@article{Tomas2003OverexpressionOG,
  title={Overexpression of groESL in Clostridium acetobutylicum results in increased solvent production and tolerance, prolonged metabolism, and changes in the cell's transcriptional program.},
  author={Christopher A. Tomas and Neil E. Welker and Eleftherios T. Papoutsakis},
  journal={Applied and environmental microbiology},
  year={2003},
  volume={69 8},
  pages={
          4951-65
        }
}
DNA array and Western analyses were used to examine the effects of groESL overexpression and host-plasmid interactions on solvent production in Clostridium acetobutylicum ATCC 824. Strain 824(pGROE1) was created to overexpress the groESL operon genes from a clostridial thiolase promoter. The growth of 824(pGROE1) was inhibited up to 85% less by a butanol challenge than that of the control strain, 824(pSOS95del). Overexpression of groESL resulted in increased final solvent titers 40% and 33… CONTINUE READING
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Plasmid effects on Escherichia coli metabolism.

Critical reviews in biotechnology • 2000
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