Overexpression of functional human FLT3 ligand in Pichia pastoris

  title={Overexpression of functional human FLT3 ligand in Pichia pastoris},
  author={Esmat Karimi and Habibollah Faraji and Daryoush Hamidi Alamdari and M. Souktanloo and Majed Mojarrad and Leonie K. Ashman and Baratali Mashkani},
  journal={Applied Biochemistry and Microbiology},
Growth factors and cytokines including FLT3 Ligand (FLT3L) have many applications in cellular and molecular biology studies, and also as therapeutical agents. FLT3L was expressed in Pichia pastoris through cloning from human leukemic K562 cell line as well as using a codon-optimized synthetic construct. Codon adaptation index (CAI) was increased from 55 in… CONTINUE READING