Overexpression of a transporter gene in a multidrug-resistant human lung cancer cell line.

@article{Cole1992OverexpressionOA,
  title={Overexpression of a transporter gene in a multidrug-resistant human lung cancer cell line.},
  author={Susan P. C. Cole and Gabu Bhardwaj and James H. Gerlach and J E Mackie and Caroline E. Grant and Kurt C. Almquist and Amanda J Stewart and Ebba U. Kurz and A. M. Duncan and R. G. Deeley},
  journal={Science},
  year={1992},
  volume={258 5088},
  pages={1650-4}
}
The doxorubicin-selected lung cancer cell line H69AR is resistant to many chemotherapeutic agents. However, like most tumor samples from individuals with this disease, it does not overexpress P-glycoprotein, a transmembrane transport protein that is dependent on adenosine triphosphate (ATP) and is associated with multidrug resistance. Complementary DNA (cDNA) clones corresponding to messenger RNAs (mRNAs) overexpressed in H69AR cells were isolated. One cDNA hybridized to an mRNA of 7.8 to 8.2… CONTINUE READING