Overexpression of Sigma Factor, ςB, Urges Staphylococcus aureus to Thicken the Cell Wall and to Resist β-Lactams

@article{Morikawa2001OverexpressionOS,
  title={Overexpression of Sigma Factor, ςB, Urges Staphylococcus aureus to Thicken the Cell Wall and to Resist $\beta$-Lactams},
  author={K. Morikawa and A. Maruyama and Y. Inose and M. Higashide and H. Hayashi and T. Ohta},
  journal={Biochemical and Biophysical Research Communications},
  year={2001},
  volume={288},
  pages={385-389}
}
Abstract Whole genome sequence analysis revealed that Staphylococcus aureus is provided with only a few sigma factors, including the alternative sigma factor, ς B , which is thought to regulate some stress responses. Since the sig B knock-out mutant did not show remarkable phenotypic difference, we constructed the over expressed mutant to examine the role of the sig B. Electron microscopic observation revealed that the mutant showed a variety of cell sizes compared with the parent strain which… Expand
Absence of Protoheme IX Farnesyltransferase CtaB Causes Virulence Attenuation but Enhances Pigment Production and Persister Survival in MRSA
TLDR
It is found that deletion of ctaB attenuated growth and virulence in mice but enhanced pigment production and formation of quinolone tolerant persister cells in stationary phase, suggesting the importance of heme biosynthesis in virulence and persister formation of S. aureus. Expand
The σB regulon in Staphylococcus aureus and its regulation
TLDR
It is shown that σ B of S. aureus, in contrast to B. subtilis, may have a function in more basic cellular processes such as cell envelope composition, membrane transport processes and intermediary metabolism. Expand
Contribution of YjbIH to Virulence Factor Expression and Host Colonization in Staphylococcus aureus
TLDR
These studies identified changes in pigmentation and protease activity in response to YjbIH and are the first to have shown a role for these proteins during infection. Expand
Functional Characterization of the σB-Dependent yabJ-spoVG Operon in Staphylococcus aureus: Role in Methicillin and Glycopeptide Resistance
TLDR
It is postulate that SpoVG is the major factor of the yabJ-spoVG operon required in S. aureus for capsule formation and antibiotic resistance. Expand
Deletion of membrane‐associated Asp23 leads to upregulation of cell wall stress genes in Staphylococcus aureus
TLDR
The asp23 gene led to an upregulation of the cell wall stress response and a function for Asp23 in cell envelope homoeostasis is suggested and identified as a membrane‐associated protein. Expand
Inactivation of the monofunctional peptidoglycan glycosyltransferase SgtB allows Staphylococcus aureus to survive in the absence of lipoteichoic acid
TLDR
It is shown that LTA is required for efficient peptidoglycan crosslinking in S. aureus and inactivation of a peptidlycercan glycosyltransferase can partially rescue this defect, altogether revealing an intimate link between peptidglycan and LTA synthesis. Expand
The YjbH Adaptor Protein Enhances Proteolysis of the Transcriptional Regulator Spx in Staphylococcus aureus
TLDR
It is concluded that the two YjbH homologues of Bacillus subtilis have a conserved physiological function and were restored to wild-type levels both under nonstress conditions and under conditions of disulfide stress. Expand
Expression of a Cryptic Secondary Sigma Factor Gene Unveils Natural Competence for DNA Transformation in Staphylococcus aureus
TLDR
A unique model for staphylococcal competence regulation by SigH is proposed that could help explain the acquisition of antibiotic resistance genes through horizontal gene transfer in this important pathogen. Expand
In vitro resistance to the CSαβ-type antimicrobial peptide ASABF-α is conferred by overexpression of sigma factor sigB in Staphylococcus aureus
TLDR
The overexpression of sigB confers resistance to the antimicrobial peptide, ASABF-a, which is particularly effective against the Gram-positive pathogen Staphylococcus aureus. Expand
Transcription of the Staphylococcus aureus cid and lrg Murein Hydrolase Regulators Is Affected by Sigma Factor B
TLDR
Results suggest that the lrgAB and cidBC genes may be part of the S. aureus sigmaB-controlled stress regulon, and that these genes are also cotranscribed as a single RNA message in early exponential growth. Expand
...
1
2
3
4
5
...

References

SHOWING 1-10 OF 18 REFERENCES
Deletion of the Alternative Sigma Factor ςB in Staphylococcus aureus Reveals Its Function as a Global Regulator of Virulence Genes
TLDR
SigmaB is a global regulator which modulates the expression of several virulence factors in S. aureus and that laboratory strain 8325 is a sigmaB-defective mutant, which was found to contain an 11-bp deletion in the regulator gene rsbU. Expand
Cell wall-affecting antibiotics induce expression of a novel gene, drp35, in Staphylococcus aureus.
TLDR
Analysis of differential hybridization with mRNAs enhanced in the presence of beta-lactams resulted in two positive clones that harbored a new gene encoding a 35,845-Da protein (Drp35) and the penicillin-binding protein 2 (PBP2) that was specific in S. aureus. Expand
Characterization of the ςB Regulon inStaphylococcus aureus
ABSTRACT The ςB-dependent stress regulon in gram-positive bacteria might fulfill a physiological role in stress response and virulence similar to that of the ςS regulon inEscherichia coli and otherExpand
Contribution of a Thickened Cell Wall and Its Glutamine Nonamidated Component to the Vancomycin Resistance Expressed by Staphylococcus aureus Mu50
ABSTRACT Staphylococcus aureus Mu50, which has reduced susceptibility to vancomycin, has a remarkably thickened cell wall with an increased proportion of glutamine nonamidated muropeptides. InExpand
Hyperproduction of Alpha-Hemolysin in asigB Mutant Is Associated with Elevated SarA Expression inStaphylococcus aureus
TLDR
It is hypothesized that inactivation of sigBleads to increased expression of SarA which, in turn, modulates target genes via an agr-independent but SarA-dependent pathway. Expand
The alternative sigma factor σB in Staphylococcus aureus: regulation of the sigB operon in response to growth phase and heat shock
TLDR
A complex transcriptional regulation that differs between S. aureus and B. subtilis is indicated, encoding an alternative sigma factor in this organism. Expand
Characterization of the Primary σ Factor of Staphylococcus aureus*
TLDR
It is concluded that plaC encodes the primary σ factor in S. aureus, and the 5′-ends of the σSA-initiated mRNAs synthesized in vitro from the agr P2 and sea promoters are in general agreement with the5′- ends of the cellular RNAs. Expand
ςB Activity Depends on RsbU inStaphylococcus aureus
TLDR
In GP268, the heat inducibility of sigma(B)-dependent genes, Asp23 production, alpha-hemolysin activity, pigmentation, and susceptibility to H2O2 were restored to the levels observed in strain Newman, clearly demonstrating that RsbU is needed for activation of s Sigma(B) in S. aureus. Expand
Identification and characterization of cell wall-cell division gene clusters in pathogenic gram-positive cocci
Clusters of peptidoglycan biosynthesis and cell division genes (DCW genes) were identified and sequenced in two gram-positive cocci, Staphylococcus aureus and Enterococcus faecalis. The resultsExpand
Themes and variations in prokaryotic cell division.
  • W. Margolin
  • Biology, Medicine
  • FEMS microbiology reviews
  • 2000
TLDR
Although some cell division determinants such as FTSZ are present in a broad spectrum of prokaryotic species, the lack of FtsZ in some species and different profiles of cell division proteins in different families suggests that there are diverse mechanisms for regulating cell division. Expand
...
1
2
...