Overexpression of Schizosaccharomyces pombe tRNA 3'-end processing enzyme Trz2 leads to an increased cellular iron level and apoptotic cell death.

  title={Overexpression of Schizosaccharomyces pombe tRNA 3'-end processing enzyme Trz2 leads to an increased cellular iron level and apoptotic cell death.},
  author={Jinjie Shang and Lin Wu and Yanmei Yang and Yan Li and Zecheng Liu and Ying Huang},
  journal={Fungal genetics and biology : FG \& B},
Multiple nutritional phenotypes of fission yeast mutants defective in genes encoding essential mitochondrial proteins
This study isolated and characterized eight temperature-sensitive (ts) strains with mutations in essential mitochondrial proteins, and promises potentially large dividends because mitochondrial quiescent functions are vital for human brain and muscle, and also for longevity.
Leucine depletion extends the lifespans of leucine-auxotrophic fission yeast by inducing Ecl1 family genes via the transcription factor Fil1
It is shown thatLeucine depletion extends the chronological lifespan (CLS) of leucine-auxotrophic cells and causes cell miniaturization and cell cycle arrest at the G1 phase, and it is proposed that these genes play important roles in the maintenance of cell survival until conditions that favor proliferation are restored.


Characterization of cis-elements in the Promoter of trz2 Encoding Schizosaccharomyces pombe Mitochondrial tRNA 3'-end Processing Enzyme.
The results suggest that the low-level trz2 expression may arise from a low level of transcription caused by lack of a strong TATA box and the NRE.
Knockout of Drosophila RNase ZL impairs mitochondrial transcript processing, respiration and cell cycle progression
Drosophila RNase ZL is implicates in a novel retrograde signaling pathway initiated by the damage in mitochondria and manifested in a cell cycle delay before the mitotic entry, suggesting a critical role for ROS.
Ectopic expression of mitochondria endonuclease Pnu1p from Schizosaccharomyces pombe induces cell death of the yeast.
Although expression of human Bak in S. pombe was lethal, Pnu1p nuclease is not necessary for hBak-induced cell death, and ectopic expression of active Pnu 1p led to cell death with fragmentation of nuclear DNA, suggesting that the Pnu2p is possibly involved in a certain type of yeast cell death via DNA fragmentation.
The Schizosaccharomyces pombe PPR protein Ppr10 associates with a novel protein Mpa1 and acts as a mitochondrial translational activator
Ppr10 functions as a general mitochondrial translational activator, likely through interaction with mitochondrial mRNAs and mitochondrial translation initiation factor Mti2, and that Ppr10 requires Mpa1 association for stability and function.
Activation of the Iron Regulon by the Yeast Aft1/Aft2 Transcription Factors Depends on Mitochondrial but Not Cytosolic Iron-Sulfur Protein Biogenesis*
Iron sensing by Aft1/Aft2 is not linked to the maturation of cytosolic/nuclear Fe-S proteins, but the mitochondrial inner membrane transporter Atm1 is important to transport the inhibitory signal.
The S. pombe mitochondrial transcriptome.
This work uncovers the complexity of a fungal mitochondrial transcriptome and provides a framework for future studies of mitochondrial gene expression using S. pombe as a model system.
Schizosaccharomyces pombe Cells Deficient in Triacylglycerols Synthesis Undergo Apoptosis upon Entry into the Stationary Phase*
This study found that two gene products (Plh1p and Dga1p) are responsible for the terminal step of TAG synthesis in the fission yeast Schizosaccharomyces pombe through two different mechanisms, and provides strong evidence suggesting that DAG, not sphingolipids, mediates fatty acids-induced lipoapoptosis in yeast.
Iron homeostasis in the fission yeast Schizosaccharomyces pombe
These regulatory responses to variations in iron concentrations ensure that iron is present within cells for essential biochemical reactions, yet prevent the accumulation of iron or iron-using proteins to deleterious levels.
The fission yeast Schizosaccharomyces pombe has two distinct tRNase Z(L)s encoded by two different genes and differentially targeted to the nucleus and mitochondria.
Evidence is provided for partitioning of tRNase Z function between two different proteins in S. pombe, although one cannot rule out specialized functions for each protein.