Overexpression of Crry protects mesangial cells from complement-mediated injury.

  title={Overexpression of Crry protects mesangial cells from complement-mediated injury.},
  author={Masaomi Nangaku and Richard J Quigg and Stuart J. Shankland and Noriko Okada and Richard J Johnson and William G. Couser},
  journal={Journal of the American Society of Nephrology : JASN},
  volume={8 2},
Crry is a membrane-associated complement regulatory protein expressed on glomerular mesangial, endothelial, and epithelial cells, which reduces C3/C5 convertase activity. This study utilized an overexpression strategy to determine the functional significance of Crry in cultured rat mesangial cells. A Crry expression vector was constructed and was tagged with a c-myc epitope that allowed transfected Crry to be distinguished from the constitutively expressed protein. In stable clones… CONTINUE READING