Overexpression of Bacterial Hemoglobin Causes Incorporation of Pref 3-Lactamase into Cytoplasmic Inclusion Bodies

@inproceedings{RINAStOverexpressionOB,
  title={Overexpression of Bacterial Hemoglobin Causes Incorporation of Pref 3-Lactamase into Cytoplasmic Inclusion Bodies},
  author={URSULA RINASt and JAMES E. BAILEYW}
}
  • URSULA RINASt, JAMES E. BAILEYW
The expression of Vitreoscilla hemoglobin (VHb) in Escherichia coli JM101(pRED2) causes the incorporation of the TEM 13-lactamase precursor into cytoplasmic inclusion bodies (IBs). Less pre-I8-lactamase is translocated and processed to its mature, periplasmic form in the strain coexpressing VHb than in the control strain E. coli JM101(pUC19) not expressing VHb. When cells are grown in a special fed-batch procedure, the formation of cytoplasmic IBs consisting of pre-13-lactamase is also… CONTINUE READING