Overexpression in Escherichia coli, purification, and characterization of Sphingomonas sp. A1 alginate lyases.

@article{Yoon2000OverexpressionIE,
  title={Overexpression in Escherichia coli, purification, and characterization of Sphingomonas sp. A1 alginate lyases.},
  author={H J Yoon and Wataru Hashimoto and Osamu Miyake and Masako Okamoto and Bunzo Mikami and Kousaku Murata},
  journal={Protein expression and purification},
  year={2000},
  volume={19 1},
  pages={84-90}
}
A bacterium Sphingomonas sp. A1 produces three kinds of alginate lyases [A1-I (66 kDa), A1-II (25 kDa), and A1-III (40 kDa)] from a single precursor, through posttranslational processing. Overexpression systems for these alginate lyases were constructed in Escherichia coli cells by controlling of the lyase genes under T7 promoter and terminator. Expression levels of A1-I, A1-II, and A1-III in E. coli cells were 3.50, 3.04, and 2.13 kU/liter of culture, respectively, and were over 10-fold higher… CONTINUE READING

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