Overexpression and purification of the particulate methane monooxygenase from Methylococcus capsulatus (Bath).

@article{Chan2011OverexpressionAP,
  title={Overexpression and purification of the particulate methane monooxygenase from Methylococcus capsulatus (Bath).},
  author={Sunney I Chan and H-Hoa T Nguyen and Kelvin H-C Chen and Steve S-F Yu},
  journal={Methods in enzymology},
  year={2011},
  volume={495},
  pages={177-93}
}
The particulate methane monooxygenase (pMMO) is a multi-copper enzyme that mediates the facile conversion of methane to methanol in methanotrophic bacteria. As a membrane-bound multi-subunit metalloprotein, the highly active protein has been difficult to isolate and purify to homogeneity for biochemical and biophysical studies. In this chapter, we describe a method to overexpress pMMO with good specific activity in high yields in the intracytoplasmic membranes of the host organism, together… CONTINUE READING