Overexpression and characterization of dark-operative protochlorophyllide reductase from Rhodobacter capsulatus.

@article{Nomata2005OverexpressionAC,
  title={Overexpression and characterization of dark-operative protochlorophyllide reductase from Rhodobacter capsulatus.},
  author={Jiro Nomata and Lee R. Swem and Carl E Bauer and Yuichi Fujita},
  journal={Biochimica et biophysica acta},
  year={2005},
  volume={1708 2},
  pages={229-37}
}
Dark-operative protochlorophyllide oxidoreductase (DPOR) plays a crucial role in light-independent (bacterio)chlorophyll biosynthesis in most photosynthetic organisms. However, the biochemical properties of DPOR are still largely undefined. Here, we constructed an overexpression system of two separable components of DPOR, L-protein (BchL) and NB-protein (BchN-BchB), in the broad-host-range vector pJRD215 in Rhodobacter capsulatus. We established a stable DPOR assay system by mixing crude… CONTINUE READING

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