Overexpression and characterization of recombinant human fusion protein IL‐6/IL‐2 (ch925)

@article{Zhao1994OverexpressionAC,
  title={Overexpression and characterization of recombinant human fusion protein IL‐6/IL‐2 (ch925)},
  author={C. Zhao and P. Tang and J. Wang and N. Mao and F. Jiang and X. Li and X. Liu and M. Zhang and Y. Ren and D. Du},
  journal={STEM CELLS},
  year={1994},
  volume={12}
}
An expression vector encoding the human recombinant fusion protein interleukin 6/interleukin 2 (IL‐6/IL‐2) was constructed. When a flexible linker had been synthesized and ligated with the IL‐2 gene fragment by polymerase chain reaction (PCR) amplification, the IL‐6 gene fragment was unidirectionally inserted into the upstream of the linker‐IL‐2 sequence. The molecule of the IL‐6‐linker‐IL‐2 fusion gene named E. coli DH5α/pfIL‐6/2 was cloned and identified by DNA sequencing. The expressed… Expand

References

SHOWING 1-10 OF 14 REFERENCES
Interleukin 2-dependent and interleukin 2-independent pathways of regulation of thymocyte function by interleukin 6.
  • J. Le, G. Fredrickson, +4 authors J. Vilcek
  • Biology, Medicine
  • Proceedings of the National Academy of Sciences of the United States of America
  • 1988
B-cell-stimulatory factor 2 (beta 2 interferon) functions as a second signal for interleukin 2 production by mature murine T cells.
Antitumor activity of recombinant interleukin 6 in mice
Interleukin 6 (IL-6) and its receptor: their role in plasma cell neoplasias.
  • T. Hirano
  • Biology, Medicine
  • International journal of cell cloning
  • 1991
...
1
2
...