Overexpression and characterization of a thermostable trehalose synthase from Meiothermus ruber

@article{Zhu2009OverexpressionAC,
  title={Overexpression and characterization of a thermostable trehalose synthase from Meiothermus ruber},
  author={Yueming Zhu and Dongsheng Wei and Jun Yu Zhang and Yufan Wang and Hengyi Xu and Laijun Xing and Mingchun Li},
  journal={Extremophiles},
  year={2009},
  volume={14},
  pages={1-8}
}
A thermostable trehalose synthase (TreS) gene from Meiothermus ruber CBS-01 was cloned and overexpressed in Escherichia coli. The purified recombinant TreS could utilize maltose to produce trehalose, and showed an optimum pH and temperature of 6.5 and 50°C, respectively. Kinetic analysis showed that the enzyme had a twofold higher catalytic efficiency (k cat/K m) for maltose than for trehalose, indicating maltose as the preferred substrate. The TreS also had a weak hydrolytic property with… CONTINUE READING

From This Paper

Figures, tables, and topics from this paper.

Citations

Publications citing this paper.
Showing 1-9 of 9 extracted citations

References

Publications referenced by this paper.
Showing 1-10 of 28 references

Cloning and expression of a trehalose synthase from Pseudomonas stutzeri CJ38 in Escherichia coli for the production of trehalose

  • JH Lee, KH Lee, +4 authors SO Chung
  • Appl Microbiol Biotechnol
  • 2005
Highly Influential
4 Excerpts

Similar Papers

Loading similar papers…