Overexpression and amplification of five genes in a multidrug-resistant Chinese hamster ovary cell line.

@article{Bliek1986OverexpressionAA,
  title={Overexpression and amplification of five genes in a multidrug-resistant Chinese hamster ovary cell line.},
  author={Alexander M van der Bliek and T van der Velde-Koerts and Victor Ling and Piet Borst},
  journal={Molecular and cellular biology},
  year={1986},
  volume={6 5},
  pages={1671-8}
}
Multidrug-resistant cells are cross-resistant to a wide range of unrelated drugs, many of which are used in cancer chemotherapy. We constructed a cDNA library from RNA of the multidrug-resistant Chinese hamster ovary cell line CHRC5. By differential screening we isolated cDNAs derived from mRNAs that are overexpressed in this cell line. The cDNAs could be grouped in five classes on the basis of transcript lengths detected in RNA blots. We infer that each class codes for a separate protein. The… CONTINUE READING