Overexpression, purification, crystallization and preliminary structural studies of p-coumaric acid decarboxylase from Lactobacillus plantarum.

@article{Rodrguez2007OverexpressionPC,
  title={Overexpression, purification, crystallization and preliminary structural studies of p-coumaric acid decarboxylase from Lactobacillus plantarum.},
  author={H{\'e}ctor Rodr{\'i}guez and Blanca de las Rivas and Rosario Palacios Mu{\~n}oz and Jos{\'e} Miguel Manche{\~n}o},
  journal={Acta crystallographica. Section F, Structural biology and crystallization communications},
  year={2007},
  volume={63 Pt 4},
  pages={300-3}
}
The substrate-inducible p-coumaric acid decarboxylase (PDC) from Lactobacillus plantarum has been overexpressed in Escherichia coli, purified and confirmed to possess decarboxylase activity. The recombinant His(6)-tagged enzyme was crystallized using the hanging-drop vapour-diffusion method from a solution containing 20%(w/v) PEG 4000, 12%(w/v) 2-propanol, 0.2 M sodium acetate, 0.1 M Tris-HCl pH 8.0 with 0.1 M barium chloride as an additive. Diffraction data were collected in-house to 2.04 A… CONTINUE READING
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