Overexpression, purification, crystallization and preliminary structural studies of catabolic ornithine transcarbamylase from Lactobacillus hilgardii.

Abstract

The catabolic ornithine transcarbamylase (cOTC; EC 2.1.3.3) from the lactic acid bacteria Lactobacillus hilgardii is a key protein involved in the degradation of arginine during malolactic fermentation. cOTC containing an N-terminal His6 tag has been overexpressed in Escherichia coli, purified and crystallized under two different experimental conditions… (More)

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@article{Rivas2007OverexpressionPC, title={Overexpression, purification, crystallization and preliminary structural studies of catabolic ornithine transcarbamylase from Lactobacillus hilgardii.}, author={Blanca de las Rivas and H{\'e}ctor Rodr{\'i}guez and I. Angulo and Rosario Muňoz and Jos{\'e} Miguel Manche{\~n}o}, journal={Acta crystallographica. Section F, Structural biology and crystallization communications}, year={2007}, volume={63 Pt 7}, pages={563-7} }