Overexpression, purification, and generation of a thermostable variant of Zymomonas mobilis fructokinase.


The gene encoding fructokinase (EC from Zymomonas mobilis has been expressed at high level in Escherichia coli by modifying the ribosome binding site using the polymerase chain reaction. A simple two-step purification from extracts of the recombinant cells results in highly purified enzyme suitable for use in fructose determination. Using the… (More)