Overexpression, purification, and crystal structure of native ER alpha LBD.

@article{Eiler2001OverexpressionPA,
  title={Overexpression, purification, and crystal structure of native ER alpha LBD.},
  author={Sylvia Eiler and Monique Gangloff and Sylvie Duclaud and Dino Moras and Marc Ruff},
  journal={Protein expression and purification},
  year={2001},
  volume={22 2},
  pages={
          165-73
        }
}
Several crystal structures of human estrogen receptor alpha ligand-binding domain (hERalpha LBD) complexed with agonist or antagonist molecules have previously been solved. The proteins had been modified in cysteine residues (carboxymethylation) or renatured in urea to circumvent aggregation and denaturation problems. In this work, high-level protein expression and purification together with crystallization screening procedure yielded high amounts of soluble protein without renaturation or… CONTINUE READING

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New directions in conventional methods of protein crystallization.

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  • 2009
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