Overcoming methodical limits of standard RHD genotyping by next-generation sequencing.

  title={Overcoming methodical limits of standard RHD genotyping by next-generation sequencing.},
  author={S Stabentheiner and Martin Danzer and Norbert Niklas and Sabine Atzm{\"u}ller and Johannes Pr{\"o}ll and Christa Hackl and Helene Polin and Katja Hofer and Christian H Gabriel},
  journal={Vox sanguinis},
  volume={100 4},
BACKGROUND AND OBJECTIVES Molecular variations of the RHD gene may result in the reduced expression of the D antigen and altered Rh phenotypes. In many occasions, they cannot be typed reliably by standard serological methods. Sequence-based typing is the gold standard to determine rare and unknown RHD genotypes. For this pilot study, sequence-based typing by standard Sanger sequencing was compared to a newly established next-generation sequencing approach based on pyrosequencing. MATERIALS… CONTINUE READING
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