Overcoming extractability hurdles of a 14C labeled taxane analogue milataxel and its metabolite from xenograft mouse tumor and brain tissues.

  title={Overcoming extractability hurdles of a 14C labeled taxane analogue milataxel and its metabolite from xenograft mouse tumor and brain tissues.},
  author={Hudan Safarpour and Paul C. Connolly and Xiaojie Tong and Mike Bielawski and Everett Wilcox},
  journal={Journal of pharmaceutical and biomedical analysis},
  volume={49 3},
Taxane analogue milataxel have been shown to bound to proteins/tissues irreversibly. Extraction of the aforementioned bound drug and metabolite was proven to be difficult task. Nonetheless, an extraction method had to be developed to accurately determine drug concentration in tissues over time. This method would enable Taxolog, Inc. (Fairfield, NJ, USA) to accurately map the fate of drug in mice and it would also enable to better design drug dosing scheme for its maximum efficiency. A… Expand
9 Citations
Bioanalysis of drug in tissue: current status and challenges.
This article provides a systematic review of tissue sample analysis for small molecules using LC-MS/MS and discusses strategies for method development, method qualification or validation, and sample analysis. Expand
Natural Products in Drug Discovery: Impacts and Opportunities — An Assessment
Nature stands as an inexhaustible source of novel chemotypes and pharmacophores, and has been a source of medicinal agents for thousands of years, and an impressive number of modern drugs find theirExpand
Bioanalysis of small and large molecule drugs, metabolites, and biomarkers by LC-MS
This book chapter highlights the bioanalysis of small and large molecule drugs, metabolites, and biomarkers by LC-MS and illustrates some of the challenges and problem-solving case examples. Expand
Natural products: an evolving role in future drug discovery.
The present review describes natural products, semi-synthetic NPs and NP-derived compounds that have undergone clinical evaluation or registration from 2005 to 2010 by disease area i.e. infectious, immunological, cardiovascular, neurological, inflammatory and related diseases and oncology. Expand
Tissue Sample Preparation in LC‐MS Bioanalysis


Bio-analysis of docetaxel and hydroxylated metabolites in human plasma by high-performance liquid chromatography and automated solid-phase extraction.
A semi-automated reversed-phase high-performance liquid chromatography method has been developed and validated for the quantification of the novel anticancer drug docetaxel in human plasma and putative hydroxylated metabolites were detected and quantified. Expand
Tumor targeting by conjugation of DHA to paclitaxel.
DHA targets pac litaxel to tumors: tumor AUCs are 61-fold higher for DHA-paclitaxel than for paclitaxe at equitoxic doses and eight-foldHigher at equimolar doses. Expand
Rapid high-performance liquid chromatographic determination of docetaxel (Taxotere) in plasma using liquid-liquid extraction.
A new rapid and sensitive high-performance liquid chromatographic method for analysis of docetaxel (Taxotere) in human plasma was developed and validated. After adding an internal standardExpand
Rapid screening of taxol metabolites in human microsomes by liquid chromatography/electrospray ionization-mass spectrometry.
Liquid chromatography with an electrospray ionization (ESI) interface has been applied to study the anticancer drug taxol and its metabolites after incubation with human hepatic microsomes, finding the presence of hydroxylated and deacetylated metabolites of taxol to be established. Expand
Sensitive determination of docetaxel in human plasma by liquid-liquid extraction and reversed-phase high-performance liquid chromatography.
A sensitive reversed-phase high-performance liquid chromatographic method has been developed and validated for the quantitative determination of docetaxel (I) in human plasma, and the accuracy, within-run and between-run precisions at three tested concentrations fell within the generally accepted criteria for bioanalytical assays. Expand
High-performance liquid chromatography tandem mass spectrometry procedure with automated solid phase extraction sample preparation for the quantitative determination of paclitaxel (Taxol) in human plasma.
A sensitive, specific, accurate and reproducible analytical method was developed and validated for the quantitation of the anticancer agent paclitaxel in human plasma. This procedure is based on highExpand