Overcoming a nucleosomal barrier to transcription

  title={Overcoming a nucleosomal barrier to transcription},
  author={Vasily M. Studitsky and David J Clark and Gary Felsenfeld},
We have studied the kinetics of transcription through a nucleosome core. RNA polymerase transcribes the first approximately 25 bp of nucleosomal DNA rapidly, but then hits a barrier and continues slowly to the nucleosomal dyad region. Here, the barrier disappears and the transcript is completed at a rapid rate, as if on free DNA, indicating that histone octamer transfer is completed as polymerase reaches the dyad. If DNA behind the polymerase is removed during transcription, the barrier does… CONTINUE READING