Outward currents in rabbit pulmonary artery cells dissociated with a new technique.

@article{Clapp1991OutwardCI,
  title={Outward currents in rabbit pulmonary artery cells dissociated with a new technique.},
  author={Lucie H Clapp and Alison M Gurney},
  journal={Experimental physiology},
  year={1991},
  volume={76 5},
  pages={677-93}
}
Single cells from the rabbit pulmonary artery were isolated using a new and convenient procedure. Strips of muscle were incubated overnight in papain at 6 degrees C and dispersed the following morning after warming the tissue for 10 min. This method consistently produced a high yield of relaxed cells, which reversibly responded to vasoconstrictors and remained viable for many hours. The electrophysiological properties of these cells were studied using the patch-clamp technique in the whole-cell… CONTINUE READING

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