Outer pore topology of the ECaC-TRPV5 channel by cysteine scan mutagenesis.

  title={Outer pore topology of the ECaC-TRPV5 channel by cysteine scan mutagenesis.},
  author={Yolaine Dodier and Umberto Banderali and H{\'e}l{\`e}ne Klein and Ozlem Topalak and Omar Dafi and Manuel Sobrinho Sim{\~o}es and G{\'e}rald Bernatchez and R{\'e}my Sauv{\'e} and Lucie Parent},
  journal={The Journal of biological chemistry},
  volume={279 8},
The substituted cysteine accessibility method (SCAM) was used to map the external vestibule and the pore region of the ECaC-TRPV5 calcium-selective channel. Cysteine residues were introduced at 44 positions from the end of S5 (Glu515) to the beginning of S6 (Ala560). Covalent modification by positively charged MTSET applied from the external medium significantly inhibited whole cell currents at 15/44 positions. Strongest inhibition was observed in the S5-linker to pore region (L520C, G521C, and… CONTINUE READING

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