Outer membrane protein AlkL boosts biocatalytic oxyfunctionalization of hydrophobic substrates in Escherichia coli.

@article{Julsing2012OuterMP,
  title={Outer membrane protein AlkL boosts biocatalytic oxyfunctionalization of hydrophobic substrates in Escherichia coli.},
  author={Mattijs Kamiel Julsing and Manfred Schrewe and Sjef Cornelissen and Inna Hermann and Andreas Schmid and Bruno B{\"u}hler},
  journal={Applied and environmental microbiology},
  year={2012},
  volume={78 16},
  pages={5724-33}
}
The outer membrane of microbial cells forms an effective barrier for hydrophobic compounds, potentially causing an uptake limitation for hydrophobic substrates. Low bioconversion activities (1.9 U g(cdw)(-1)) have been observed for the ω-oxyfunctionalization of dodecanoic acid methyl ester by recombinant Escherichia coli containing the alkane monooxygenase AlkBGT of Pseudomonas putida GPo1. Using fatty acid methyl ester oxygenation as the model reaction, this study investigated strategies to… CONTINUE READING

Citations

Publications citing this paper.
Showing 1-10 of 20 extracted citations

References

Publications referenced by this paper.
Showing 1-10 of 74 references

Kinetic analysis of terminal and unactivated C-H bond oxyfunctionalization in fatty acid methyl esters by monooxygenase-based whole-cell biocatalysis

  • M Schrewe, A Magnusson, C Willrodt, B Bühler, A. Schmid
  • Adv . Synth . Catal .
  • 2011
Highly Influential
10 Excerpts

High cell density fermentations of Pseudomonas oleovorans for the production of mcl-PHAs in two-liquid phase media

  • MB Kellerhals, W Hazenberg, B. Witholt
  • Enzyme Microb. Technol
  • 1999
Highly Influential
3 Excerpts

Intensification and economic and ecological assessment of a biocatalytic oxyfunctionalization process

  • D Kuhn, MA Kholiq, E Heinzle, B Bühler, A. Schmid
  • Green Chem
  • 2010
1 Excerpt

Similar Papers

Loading similar papers…