Ouabain binding to renal tubules of the rabbit

@article{Shaver1978OuabainBT,
  title={Ouabain binding to renal tubules of the rabbit},
  author={Joan L F Shaver and Colin Stirling},
  journal={The Journal of Cell Biology},
  year={1978},
  volume={76},
  pages={278 - 292}
}
It is well known that ouabain, a specific inhibitor of Na-K ATPase-dependent transport, interferes with renal tubular salt reabsorption. In this study, we employed radiochemical methods to measure the kinetics of [3H]ouabain binding to slices of rabbit renal medulla and high resolution quantitative autoradiography to determine the location and number of cellular binding sites. The kinetics obeyed a simple bimolecular reaction with an association constant of 2.86 +/- 0.63 SD x 10(3) M-1 min-1… CONTINUE READING
18 Citations
71 References
Similar Papers

Citations

Publications citing this paper.
Showing 1-10 of 18 extracted citations

References

Publications referenced by this paper.
Showing 1-10 of 71 references

Ion move- ments and oxygen consumption in kidney cortex slices

  • R. WHrrrAM, J. S. WILLIS
  • J, Physiol. (Lond.)
  • 1963
Highly Influential
3 Excerpts

Cellular location and mem- brane density of Na-K ATPase and their relation- ship to ion transport

  • C. E. STIRLING
  • 1978
1 Excerpt

Similar Papers

Loading similar papers…