Osteotropic factor responsiveness of highly purified populations of early and late precursors for human multinucleated cells expressing the osteoclast phenotype.

@article{Kurihara1991OsteotropicFR,
  title={Osteotropic factor responsiveness of highly purified populations of early and late precursors for human multinucleated cells expressing the osteoclast phenotype.},
  author={Noriyoshi Kurihara and C J Civin and Garson David Roodman},
  journal={Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research},
  year={1991},
  volume={6 3},
  pages={257-61}
}
Recently we have adapted human long-term bone marrow cultures to form multinucleated cells (MNC) that express the osteoclast phenotype and used semisolid culture techniques to identify early (bipotent) and late (unipotent) mononuclear precursors for these MNC. The early precursor can form both osteoclast-like MNC and macrophage polykaryons; the late precursor forms only osteoclast-like MNC. In this study we examined the effects of osteotropic hormones and cytokines of MNC formation from highly… CONTINUE READING