Osteopontin knockdown suppresses the growth and angiogenesis of colon cancer cells.

@article{Wu2014OsteopontinKS,
  title={Osteopontin knockdown suppresses the growth and angiogenesis of colon cancer cells.},
  author={Xin-lin Wu and Kai-jin Lin and Ai-ping Bai and Wan-xiang Wang and Xing-kai Meng and Xiu-lan Su and Ming-xing Hou and Pei-de Dong and J Zhang and Zhaoyang Wang and Lin Shi},
  journal={World journal of gastroenterology},
  year={2014},
  volume={20 30},
  pages={10440-8}
}
AIM To investigate the effects of osteopontin (OPN) gene expression knockdown on colon cancer Lovo cells in vitro. METHODS Four candidate small interfering RNA (siRNA) constructs targeting the OPN gene and a scrambled control sequence (NC-siRNA) were synthesized and inserted into a pGPU6/GFP/Neo expression vector. After confirmation by restriction enzyme digestion and DNA sequencing, the recombinant plasmids were subsequently transfected into a human colon cancer cell line (Lovo) using a… CONTINUE READING
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