Osteopontin improves sperm capacitation and in vitro fertilization efficiency in buffalo (Bubalus bubalis).

@article{Boccia2013OsteopontinIS,
  title={Osteopontin improves sperm capacitation and in vitro fertilization efficiency in buffalo (Bubalus bubalis).},
  author={Lucia Boccia and Serena Di Francesco and Gianluca Neglia and Marina De Blasi and Valentina Longobardi and Giuseppe Campanile and Bianca Gasparrini},
  journal={Theriogenology},
  year={2013},
  volume={80 3},
  pages={212-7}
}
The aim of this study was to evaluate the effect of osteopontin (OPN), an ubiquitous acid glycoprotein, on in vitro sperm capacitation and on in vitro embryo production (IVEP) efficiency in buffalo. In experiment 1, after swim-up separation the sperm were incubated in Tyrode albumin lactate pyruvate medium in the absence of capacitating agents (control), with the standard concentration of heparin (0.01 mM) and three different concentrations of OPN (0.1, 1, and 10 mcg/mL), both in the presence… CONTINUE READING