Osmotic tolerance limits and properties of murine spermatozoa.

  title={Osmotic tolerance limits and properties of murine spermatozoa.},
  author={Colin Eric Willoughby and Peter Mazur and Augustine T Peter and John K. Critser},
  journal={Biology of reproduction},
  volume={55 3},
Osmotic tolerance of spermatozoa is a critical determinant of functional survival after cryopreservation. This study first tested the hypothesis that mouse spermatozoa behave as linear osmometers, using an electronic particle counter to measure the change in sperm volume in response to anisosmotic solutions. The resulting Boyle-van't Hoff plot was linear (r2 = 0.99) from 75 to 1200 mOsmolal and indicates that 60.7% of the total cell volume is osmotically inactive. Next, mouse sperm tolerance to… CONTINUE READING


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