Osmotic responses and tolerance limits to changes in external osmolalities, and oolemma permeability characteristics, of human in vitro matured MII oocytes.

@article{Abbeel2007OsmoticRA,
  title={Osmotic responses and tolerance limits to changes in external osmolalities, and oolemma permeability characteristics, of human in vitro matured MII oocytes.},
  author={Etienne van den Abbeel and Ulrich Schneider and Jun Liu and Yuksel Agca and John Kenneth Critser and Andr{\'e} van Steirteghem},
  journal={Human reproduction},
  year={2007},
  volume={22 7},
  pages={
          1959-72
        }
}
BACKGROUND Oocyte cryopreservation remains a realistic objective, provided that more systematic approaches are applied, such as thorough analysis of the oocyte oolemma permeability to water and diverse cryoprotectants. METHODS We prospectively investigated volume changes over time at different temperatures (30 degrees C, 22 degrees C and 8 degrees C) of human metaphase II (MII) oocytes (obtained in stimulated ICSI cycles and matured in vitro from the germinal vesicle stage) when exposed to… CONTINUE READING

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