Osmotic and curvature stress affect PEG-induced fusion of lipid vesicles but not mixing of their lipids.

@article{Malinin2002OsmoticAC,
  title={Osmotic and curvature stress affect PEG-induced fusion of lipid vesicles but not mixing of their lipids.},
  author={Vladimir S Malinin and Peter M. Frederik and Barry R. Lentz},
  journal={Biophysical journal},
  year={2002},
  volume={82 4},
  pages={2090-100}
}
Poly (ethylene glycol) (PEG) in the external environment of membrane vesicles creates osmotic imbalance that leads to mechanical stress in membranes and may induce local membrane curvature. To determine the relative importance of membrane stress and curvature in promoting fusion, we monitored contents mixing (CM) and lipid mixing (LM) between different sized vesicles under a variety of osmotic conditions. CM between highly curved vesicles (SUV, 26 nm diameter) was up to 10 times greater than… CONTINUE READING