Osmotic Response Element Enhancer Activity

@article{Nadkarni1999OsmoticRE,
  title={Osmotic Response Element Enhancer Activity},
  author={Varsha Nadkarni and Kenneth H. Gabbay and Kurt M. Bohren and David Sheikh-Hamad},
  journal={The Journal of Biological Chemistry},
  year={1999},
  volume={274},
  pages={20185 - 20190}
}
Hypertonicity induces a group of genes that are responsible for the intracellular accumulation of protective organic osmolytes such as sorbitol and betaine. Two representative genes are the aldose reductase enzyme (AR, EC 1.1.1.21), which is responsible for the conversion of glucose to sorbitol, and the betaine transporter (BGT1), which mediates Na+-coupled betaine uptake in response to osmotic stress. We recently reported that the induction of BGT1 mRNA in the renal epithelial Madin-Darby… Expand
Hyperosmotic Stress Stimulates Promoter Activity and Regulates Cellular Utilization of the Serum- and Glucocorticoid-inducible Protein Kinase (Sgk) by a p38 MAPK-dependent Pathway*
TLDR
It is proposed that the stimulated expression of enzymatically active Sgk after sorbitol treatment is a newly defined component of the p38 MAPK-mediated response to hyperosmotic stress. Expand
Aldose Reductase Induced by Hyperosmotic Stress Mediates Cardiomyocyte Apoptosis
TLDR
The data show that hypertonic shrinkage of cardiac myocytes alone is not sufficient to induce cardiac myocyte apoptosis, and hyperosmolarity-induced cell death is sensitive to the nature of the osmolyte and requires induction of aldose reductase as well as a decrease in intracellular glutathione levels. Expand
Cyclosporine A inhibits the adaptive responses to hypertonicity: a potential mechanism of nephrotoxicity.
TLDR
It is shown that cyclosporine A prevents the nuclear translocation of the transcription nuclear factor of activated T lymphocyte 5 and inhibits osmotic response element-mediated reporter gene expression and Histologic examination of the kidneys of CsA-treated rats shows a marked increase in apoptosis in the renal medulla where hypertonicity normally prevails. Expand
Fyn and p38 Signaling Are Both Required for Maximal Hypertonic Activation of the Osmotic Response Element-binding Protein/Tonicity-responsive Enhancer-binding Protein (OREBP/TonEBP)*
  • B. Ko, A. Lam, A. Kapus, Lingzhi Fan, Sookja K. Chung, Stephen S. M. Chung
  • Biology, Medicine
  • The Journal of Biological Chemistry
  • 2002
TLDR
It is shown that the transactivation domain of OREBP/TonEBP is the target of p38- and Fyn-mediated hypertonic activation, indicating a dual control in regulating the expression of the osmoprotective genes in mammalian cells. Expand
Involvement of multiple kinase pathways in stimulation of gene transcription by hypertonicity.
TLDR
12 genes whose mRNA expression is significantly increased within 4 h after exposure tohypertonicity are reported, supporting the notion that hypertonicity evokes the stress response and growth regulation in cells. Expand
Regulatory function of hyperosmotic stress-induced signaling cascades in the expression of transcription factors and osmolyte transporters in freshwater Japanese eel primary gill cell culture
TLDR
Collectively, the present study reveals possible functional links of osmosensing signaling cascades to the regulation of downstream effectors. Expand
TonEBP transcriptional activator in the cellular response to increased osmolality
TLDR
TonEBP plays a significant role in immunity by stimulating several cytokine genes in the T-cells by regulating changes in diuretic status mainly at the level of nucleocytoplasmic distribution. Expand
Hypertonicity-induced phosphorylation and nuclear localization of the transcription factor TonEBP.
TLDR
It is reported that hypertonicity induces an increase in TonEBP phosphorylation that temporally correlates with increased nuclear localization of the molecule, an early step in the response of cells tohypertonicity and may be required for nuclear import or retention. Expand
The Transcriptional Response of Saccharomyces cerevisiae to Osmotic Shock
TLDR
The expression of genes that are known to be controlled by the mating pheromone response pathway was stimulated by osmotic shock specifically in a hog1 mutant, suggesting that Msn2p/Msn4p might be regulated by the HOG pathway. Expand
p38 MAPK mediates acid-induced transcription of PEPCK in LLC-PK(1)-FBPase(+) cells.
TLDR
It is concluded that the SB-203580-sensitive p38alpha-ATF-2 signaling pathway is a likely mediator of the pH-responsive induction of PEPCK mRNA levels in renal LLC-PK(1)-FBPase(+) cells. Expand
...
1
2
3
4
5
...

References

SHOWING 1-10 OF 32 REFERENCES
Identification and Characterization of Multiple Osmotic Response Sequences in the Human Aldose Reductase Gene*
TLDR
The data indicate that cooperative interaction among the three TonE-like sequences in the human AR may be necessary for their enhancer function, suggesting that the mechanism of osmotic regulation of gene expression in these animals is similar. Expand
Characterization of the osmotic response element of the human aldose reductase gene promoter.
  • B. Ruepp, K. Bohren, K. Gabbay
  • Medicine, Biology
  • Proceedings of the National Academy of Sciences of the United States of America
  • 1996
TLDR
Findings will permit future studies to identify the transcription factors involved in the normal regulatory response mechanism to hypertonicity and to identify whether and how this response is altered in a variety of pathologic states, including diabetes. Expand
The tonicity-sensitive element that mediates increased transcription of the betaine transporter gene in response to hypertonic stress.
TLDR
To investigate the molecular mechanism of transcriptional regulation by tonicity, the 5'-flanking region of the gene is characterized and a smaller region that is required for hypertonicity to induce increased expression and a slowly migrating band on mobility shift assays is identified. Expand
Tonicity-responsive enhancer binding protein, a rel-like protein that stimulates transcription in response to hypertonicity.
TLDR
CDNA cloning of human TonE binding protein (TonEBP) is reported, a transcription factor that stimulates transcription through its binding to TonE sequences via a Rel-like DNA binding domain that plays a key role in hypertonicity-induced transcriptional stimulation of SMIT, sodium/chloride/betaine cotransporter, and aldose reductase. Expand
Distinct Regulation of Osmoprotective Genes in Yeast and Mammals
TLDR
Whether p38 or SAPK/JNK signal synthesis of the osmoprotectant sorbitol in rabbit renal medullary cells (PAP-HT25), analogous to the glycerol system in yeast, is asked and hyperosmolality is concluded, analogous to inducing the HOG1 cascade in yeast. Expand
ORE, a Eukaryotic Minimal Essential Osmotic Response Element
TLDR
This work functionally defined the smallest sequence able to confer hyperosmotic response on a downstream gene independent of other putative cis-elements, that is, a minimal essential osmotic response element (ORE). Expand
p38 Kinase Activity Is Essential for Osmotic Induction of mRNAs for HSP70 and Transporter for Organic Solute Betaine in Madin-Darby Canine Kidney Cells*
TLDR
It is shown that inhibition of p38 kinase activity, using the specific inhibitor SB203580, abolishes the hypertonicity-mediated induction of mRNAs for HSP70 and betaine transporter in Madin-Darby canine kidney cells and that the increased activity of JNK-1 during p38Kinase inhibition is consistent with regulation of J NK-1 by p38 Kinase in osmotically stressed cells. Expand
Molecular cloning of cDNA coding for kidney aldose reductase. Regulation of specific mRNA accumulation by NaCl-mediated osmotic stress.
TLDR
The finding of increased abundance of a specific mRNA in direct response to hyperosmotic stress represents the first report of such an effect in animals. Expand
Osmotic regulation of aldose reductase protein synthesis in renal medullary cells.
TLDR
The osmoregulatory increase in aldose reductase protein is due to an increase in its synthesis rate and not to any change in its degradation, which is not affected by osmolality. Expand
The MAP kinase cascade is not essential for transcriptional stimulation of osmolyte transporter genes.
TLDR
Evaluating the importance of the mitogen-activated protein kinase pathway in transcriptional activation of the genes for the two osmolytes cotransporters found it to be essential for the osmo-protective transcriptional response of yeast to hypertonicity. Expand
...
1
2
3
4
...