Osmostress response in Bacillus subtilis: characterization of a proline uptake system (OpuE) regulated by high osmolarity and the alternative transcription factor sigma B.

@article{Blohn1997OsmostressRI,
  title={Osmostress response in Bacillus subtilis: characterization of a proline uptake system (OpuE) regulated by high osmolarity and the alternative transcription factor sigma B.},
  author={C von Blohn and Bettina Kempf and R M Kappes and Erhard Bremer},
  journal={Molecular microbiology},
  year={1997},
  volume={25 1},
  pages={175-87}
}
Exogenously provided proline has been shown to serve as an osmoprotectant in Bacillus subtilis. Uptake of proline is under osmotic control and functions independently of the known transport systems for the osmoprotectant glycine betaine. We cloned the structural gene (opuE) for this proline transport system and constructed a chromosomal opuE mutant by marker replacement. The resulting B. subtilis strain was entirely deficient in osmoregulated proline transport activity and was no longer… CONTINUE READING

6 Figures & Tables

Connections & Topics

Mentioned Connections BETA
This osmoregulated proline transport activity was entirely dependent on de novo protein synthesis , suggesting a transcriptional control mechanism .
This osmoregulated proline transport activity was entirely dependent on de novo protein synthesis , suggesting a transcriptional control mechanism .
This osmoregulated proline transport activity was entirely dependent on de novo protein synthesis , suggesting a transcriptional control mechanism .
This osmoregulated proline transport activity was entirely dependent on de novo protein synthesis , suggesting a transcriptional control mechanism .
Database searches indicated that OpuE is a member of the sodium / solute symporter family , comprising proteins from both prokaryotes and eukaryotes that obligatorily couple substrate uptake to Na+ symport .
Database searches indicated that OpuE is a member of the sodium / solute symporter family , comprising proteins from both prokaryotes and eukaryotes that obligatorily couple substrate uptake to Na+ symport .
All Topics