Oscillographic Polarography as a Method for Continuous Measurements of Inactivation of Penicillins by Penicillinase

THE penicillins are enzymatically inactivated by penicillinase, which splits the β-lactam configuration producing penicilloic acids1. This process has so far been followed indirectly by manometric2, alkalimetric3, polarimetric4, iodometric5 and lengthy microbiological methods6,7.