Orthologous CRISPR/Cas9 systems for specific and efficient degradation of covalently closed circular DNA of hepatitis B virus

@article{Kostyushev2019OrthologousCS,
  title={Orthologous CRISPR/Cas9 systems for specific and efficient degradation of covalently closed circular DNA of hepatitis B virus},
  author={Dmitry Kostyushev and Sergey Brezgin and Anastasiya Kostyusheva and Dmitry Zarifyan and Irina A. Goptar and Vladimir Chulanov},
  journal={Cellular and Molecular Life Sciences},
  year={2019},
  volume={76},
  pages={1779-1794}
}
Covalently closed circular DNA (cccDNA) of hepatitis B virus (HBV) is the major cause of viral persistence and chronic hepatitis B. CRISPR/Cas9 nucleases can specifically target HBV cccDNA for decay, but off-target effects of nucleases in the human genome limit their clinical utility. CRISPR/Cas9 systems from four different species were co-expressed in cell lines with guide RNAs targeting conserved regions of the HBV genome. CRISPR/Cas9 systems from Streptococcus pyogenes (Sp) and Streptococcus… CONTINUE READING
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